| Autor: |
Korshun LN, Moĭsa LN, Ganova LA, Vudmaska MI, Kovtoniuk GV, Kiseleva EK, Spivak NIa |
| Jazyk: |
ruština |
| Zdroj: |
Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993) [Mikrobiol Z] 2011 Sep-Oct; Vol. 73 (5), pp. 36-46. |
| Abstrakt: |
It is shown that the recombinant protein GST-HSV2gG, containing the immunodominant regions of glycoprotein G of HSV-2 is accumulated in the form of inclusion bodies or in soluble form in the Escherichia coli BL21 (DE3) cells. The ratio between protein fractions varied depending on the physiological state of cells before biosynthesis. The kinetic parameters of bacterial populations were determined by mathematical modeling of growth curves based on the Verhulst logistic function. It was established that the induction of biosynthesis in the growth acceleration phase (at OD600 = 0.3) with 0.1 mM IPTG gives the maximum yield of soluble protein (26.75 mg/l or 17.6 mg/g biomass). The target protein was purified using the immobilized metal ion affinity and affinity chromatography technologies. Antigenic activity of the soluble form of recombinant protein GST-HSV2gG, was significantly (three times) higher than that of the protein purified from inclusion bodies (p < 0.05) and was comparable with the activity of the commercial analog (p > 0.05), that allows using this product in the immunosorbent test kits for diagnosis of IgG to HSV-2. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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