| Autor: |
Xu JL; UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China., Zhang X, Sun HY, Chi ZM |
| Jazyk: |
angličtina |
| Zdroj: |
Marine biotechnology (New York, N.Y.) [Mar Biotechnol (NY)] 2012 Jun; Vol. 14 (3), pp. 261-9. Date of Electronic Publication: 2011 Oct 08. |
| DOI: |
10.1007/s10126-011-9409-0 |
| Abstrakt: |
As the β-1, 3-glucanase produced by the marine-derived Williopsis saturnus WC91-2 could inhibit the activity of the killer toxin produced by the same yeast, the WsEXG1 gene encoding exo-β-1, 3-glucanase in W. saturnus WC91-2 was disrupted. The disruptant WC91-2-2 only produced a trace amount of β-1, 3-glucanase but had much higher activity of killer toxin than W. saturnus WC91-2. After the disruption of the WsEXG1 gene, the expression of the gene was significantly decreased from 100% in the cells of W. saturnus WC91-2 to 27% in the cells of the disruptant WC91-2-2 while the expression of the killer toxin gene in W. saturnus WC91-2 and the disruptant WC91-2-2 was almost the same. During 2-l fermentation, the disruptant WC91-2-2 could produce the highest amount of killer toxin (the size of the inhibition zone was 22 ± 0.7 mm) within 36 h when the cell growth reached the middle of the log phase. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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