| Autor: |
Almeida-Val VM; Laboratório de Ecofisiologia e Evolução Molecular, Instituto Nacional de Pesquisas da Amazônia, Manaus, AM, Brazil., Oliveira AR, de Nazaré Paula da Silva M, Ferreira-Nozawa MS, Araújo RM, Val AL, Nozawa SR |
| Jazyk: |
angličtina |
| Zdroj: |
Genetics and molecular biology [Genet Mol Biol] 2011 Apr; Vol. 34 (2), pp. 315-22. Date of Electronic Publication: 2011 Apr 01. |
| DOI: |
10.1590/s1415-47572011000200025 |
| Abstrakt: |
Adaptation or acclimation to hypoxia occurs via the modulation of physiologically relevant genes, such as erythropoietin, transferrin, vascular endothelial growth factor, phosphofructokinase and lactate dehydrogenase A. In the present study, we have cloned, sequenced and examined the modulation of the LDH-A gene after an Amazonian fish species, Astronotus crassipinis (the Oscar), was exposed to hypoxia and anoxia. In earlier studies, we have discovered that adults of this species are extremely tolerant to hypoxia and anoxia, while the juveniles are less tolerant. Exposure of juveniles to acute hypoxia and anoxia resulted in increased LDH-A gene expression in skeletal and cardiac muscles. When exposed to graded hypoxia juveniles show decreased LDH-A expression. In adults, the levels of LDH-A mRNA did not increase in hypoxic or anoxic conditions. Our results demonstrate that, when given time for acclimation, fish at different life-stages are able to respond differently to survive hypoxic episodes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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