| Autor: |
Kaĭsheva AL, Ivanov IuD, Zgoda VG, Frantsuzov PA, Pleshakova TO, Krokhin NV, Ziborov VS, Archakov AI |
| Jazyk: |
ruština |
| Zdroj: |
Biomeditsinskaia khimiia [Biomed Khim] 2010 Jan-Feb; Vol. 56 (1), pp. 26-39. |
| DOI: |
10.18097/pbmc20105601026 |
| Abstrakt: |
Possibility of detection and identification of hepatitis C viral particles with mass spectrometry (MS) in combination with atomic force microscopy (AFM) had been investigated. AFM/MS approach is based on two technologies: (1) AFM-biospecific fishing that allows to detect, concentrate from solution and to count protein complexes on a surface of AFM-nanochip; (2) mass spectrometric identification of these complexes. AFM-biospecific fishing of HCVcoreAg from solution was carried onto surface of AFM-nanochips with immobilized anti-HCVcoreAg. It was shown that HCVcoreAg/anti-HCVcore(im) complexes were formed onto AFM-nanochips in quantity sufficient for mass spectrometric identification. Thus, AFM/MS approach allows to identify fragments of hepatitis C virus fished onto a surface of AFM-nanochip from serum. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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