| Autor: |
Bai Y; The state key Lab of Agrobiotechnology National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Li YR, Wang GH, Zhou XM, Zhao DM |
| Jazyk: |
angličtina |
| Zdroj: |
Virologica Sinica [Virol Sin] 2010 Dec; Vol. 25 (6), pp. 440-4. Date of Electronic Publication: 2011 Jan 10. |
| DOI: |
10.1007/s12250-010-3143-z |
| Abstrakt: |
Prion diseases are infectious and fatal neurodegenerative diseases. The pathogenic agent is an abnormal prion protein aggregate. Microglial activation in the centre nervous system is a characteristic feature of prion disease. In this study, we examined the effect of PrP 106-126 on PrP mRNA gene expression in Mouse microglia cells BV-2 by real-time quantitative PCR. PrP mRNA expression level was found to be significantly increased after 18 h exposure of BV-2 cells to PrP 106-126, with 3-fold increase after 18 h and 4.5-fold increase after 24 h and BV-2 cells proliferating occurred correspondingly. Our results provide the first in vitro evidence of the increase of PrP mRNA levels in microglial cells exposed to PrP 106-126, and indicate that microglial cells might play a critical role in prion pathogenesis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Full text from SpringerLink