Energy-dependent collision-induced dissociation study of buprenorphine and its synthetic precursors.

Autor: Biri B; Department of Applied Chemistry, University of Debrecen, H-4032 Debrecen, Egyetem tér 1, Hungary., Kalmár J, Nagy L, Sipos A, Zsuga M, Kéki S
Jazyk: angličtina
Zdroj: Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 2011 Jan 15; Vol. 25 (1), pp. 41-9.
DOI: 10.1002/rcm.4821
Abstrakt: The collision-induced dissociation (CID) of protonated buprenorphine ([M+H](+) ) and four related compounds was studied by electrospray quadrupole/time-of-flight mass spectrometry (ESI-QTOF MS). The fragmentation pathways were investigated by using energy-dependent CID and pseudo-MS(3) (in-source CID combined with tandem mass spectrometry (MS/MS)) methods. The first steps of the fragmentation are the parallel losses of the substituents from the non-aromatic ring moieties. Depending on the applied collision energies, a large number of further fragment ions arising from the cross-ring cleavages of the core-ring structure were observed. Based on the experimental results, a generalized fragmentation scheme was developed for the five buprenorphine derivatives highlighting the differences for the alternatively substituted compounds. The collision-energy-dependent fragmentation profile of buprenorphine is visualized in a two-dimensional plot to aid its fingerprint identification.
(Copyright © 2010 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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