[Identification by PCR and antifungal susceptibility of vaginal clinical Candida sp isolates].

Autor:	Alves IA; Curso de Farmácia Bioquímica Clínica, Universidade Regional Integrada do Alto Uruguai e das Missões, Santo Ângelo, RS. izabelalmeida@hotmail.com, Camargo FP, Goulart LS
Jazyk:	portugalština
Zdroj:	Revista da Sociedade Brasileira de Medicina Tropical [Rev Soc Bras Med Trop] 2010 Sep-Oct; Vol. 43 (5), pp. 575-9.
DOI:	10.1590/s0037-86822010000500021
Abstrakt:	Introduction: Vaginal candidiasis is a condition that affects innumerous fertile women. Candida albicans is the most frequently isolated species from vaginal discharge; however, other different species that are more resistant to antifungal drugs can be identified in vaginal clinical samples. Methods: The species of 30 vaginal Candida isolates was identified by PCR using the universal ITS4 primer and species-specific primers for C. albicans, C. glabrata, C. tropicalis and C. krusei. The sensitivity pattern to amphotericin B, fluconazole and voriconazole was assessed using the CLSI M27-A2 macrodilution method. Results: The PCR assay revealed 28 C. albicans and 2 samples showed amplification for C. albicans and C. glabrata primers. The minimum inhibitory concentration for amphotericin B ranged from 0.03 µg/mL to 0.25 µg/mL, for fluconazole from 0.125 µg/ml to 16 µg/mL and for voriconazole from 0.03 µg/mL to 0.25 µg/m. Conclusions: Identification at Candida species level by PCR assay could be relevant for clinical management of these infections.
Databáze:	MEDLINE
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