| Autor: |
Essaka DC; Department of Chemistry, University of Washington, Box 351700, Seattle, Washington 98195, USA., White J, Rathod P, Whitmore CD, Hindsgaul O, Palcic MM, Dovichi NJ |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical chemistry [Anal Chem] 2010 Dec 01; Vol. 82 (23), pp. 9955-8. Date of Electronic Publication: 2010 Nov 02. |
| DOI: |
10.1021/ac1021776 |
| Abstrakt: |
The metabolism of glycosphingolipids by the malaria-causing parasite Plasmodium falciparum plays an important role in the progression of the disease. We report a new and highly sensitive method to monitor the uptake of glycosphingolipids in infected red blood cells (iRBCs). A tetramethylrhodamine-labeled glycosphingolipid (GM1-TMR) was used as a substrate. Uptake was demonstrated by fluorescence microscopy. The iRBCs were lysed with a 15% solution of saponin and washed with phosphate buffered saline to release intact parasites. The parasites were further lysed and the resulting homogenates were analyzed by capillary electrophoresis with laser-induced fluorescence detection. The lysate from erythrocytes infected at 1% parasitemia generated a signal 20 standard deviations larger than uninfected erythrocytes, which suggests that relatively low infection levels can be studied with this technique. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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