| Autor: |
Bhartia R; Jet Propulsion Laboratory, California Institute of Technology, 4800 Oak Grove Drive, M/S 183-301, Pasadena, CA 91109, USA. rbhartia@jpl.nasa.gov, Salas EC, Hug WF, Reid RD, Lane AL, Edwards KJ, Nealson KH |
| Jazyk: |
angličtina |
| Zdroj: |
Applied and environmental microbiology [Appl Environ Microbiol] 2010 Nov; Vol. 76 (21), pp. 7231-7. Date of Electronic Publication: 2010 Sep 03. |
| DOI: |
10.1128/AEM.00943-10 |
| Abstrakt: |
We introduce a near-real-time optical imaging method that works via the detection of the intrinsic fluorescence of life forms upon excitation by deep-UV (DUV) illumination. A DUV (<250-nm) source enables the detection of microbes in their native state on natural materials, avoiding background autofluorescence and without the need for fluorescent dyes or tags. We demonstrate that DUV-laser-induced native fluorescence can detect bacteria on opaque surfaces at spatial scales ranging from tens of centimeters to micrometers and from communities to single cells. Given exposure times of 100 μs and low excitation intensities, this technique enables rapid imaging of bacterial communities and cells without irreversible sample alteration or destruction. We also demonstrate the first noninvasive detection of bacteria on in situ-incubated environmental experimental samples from the deep ocean (Lo'ihi Seamount), showing the use of DUV native fluorescence for in situ detection in the deep biosphere and other nutrient-limited environments. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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