Immunization of neonatal mice with LAMP/p55 HIV gag DNA elicits robust immune responses that last to adulthood.

Autor: Rigato PO; Laboratory of Dermatology and Immunodeficiency, LIM-56, School of Medicine, University of São Paulo, Brazil., Maciel M Jr, Goldoni AL, Piubelli O, de Brito CA, Fusaro AE, Eurico de Alencar LX, August T, Azevedo Marques ET Jr, da Silva Duarte AJ, Sato MN
Jazyk: angličtina
Zdroj: Virology [Virology] 2010 Oct 10; Vol. 406 (1), pp. 37-47. Date of Electronic Publication: 2010 Jul 29.
DOI: 10.1016/j.virol.2010.06.050
Abstrakt: Successful T cell priming in early postnatal life that can generate effective long-lasting responses until adulthood is critical in HIV vaccination strategies because it prevents early sexual initiation and breastfeeding transmission of HIV. A chimeric DNA vaccine encoding p55 HIV gag associated with lysosome-associated membrane protein 1 (LAMP-1; which drives the antigen to the MIIC compartment), has been used to enhance cellular and humoral antigen-specific responses in adult mice and macaques. Herein, we investigated LAMP-1/gag vaccine immunogenicity in the neonatal period in mice and its ability to generate long-lasting effects. Neonatal vaccination with chimeric LAMP/gag generated stronger Gag-specific immune responses, as measured by the breadth of the Gag peptide-specific IFN-gamma, proliferative responsiveness, cytokine production and antibody production, all of which revealed activation of CD4+ T cells as well as the generation of a more robust CTL response compared to gag vaccine alone. To induce long-lived T and B cell memory responses, it was necessary to immunize neonates with the chimeric LAMP/gag DNA vaccine. The LAMP/gag DNA vaccine strategy could be particularly useful for generating an anti-HIV immune response in the early postnatal period capable of inducing long-term immunological memory.
(Copyright © 2010 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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