Trypanosoma cruzi: identification of DNA targets of the nuclear periphery coiled-coil protein TcNUP-1.

Autor: Picchi GF; Fundação Oswaldo Cruz, Instituto Carlos Chagas, R Professor Algacyr Munhoz Mader, 3775 Cidade Industrial-Paraná, 81350-010 Curitiba, PR, Brazil. gpicchi@tecpar.br, Ferreira AM, Souza FS, Lourenço EE, Arauco PR, Lorusso A, Bordignon J, Krieger MA, Goldenberg S, Fragoso SP
Jazyk: angličtina
Zdroj: Experimental parasitology [Exp Parasitol] 2011 Jan; Vol. 127 (1), pp. 147-52. Date of Electronic Publication: 2010 Jul 18.
DOI: 10.1016/j.exppara.2010.07.009
Abstrakt: The nuclear lamina is a structure that lines the inner nuclear membrane. In metazoans, lamins are the primary structural components of the nuclear lamina and are involved in several processes. Eukaryotes that lack lamins have distinct proteins with homologous functions. Some years ago, a coiled-coil protein in Trypanosoma brucei, NUP-1, was identified as the major filamentous component of its nuclear lamina. However, its precise role has not been determined. We characterized a homologous protein in Trypanosoma cruzi, TcNUP-1, and identified its in vivo DNA binding sites using a chromatin immunoprecipitation assay. We demonstrate for the first time that TcNUP-1 associates with chromosomal regions containing large non-tandem arrays of genes encoding surface proteins. We therefore suggest that TcNUP-1 is a structural protein that plays an essential role in nuclear organization by anchoring T. cruzi chromosomes to the nuclear envelope.
(Copyright © 2010 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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