Characterization of atypical scrapie cases from Great Britain in transgenic ovine PrP mice.

Autor:	Griffiths PC; Molecular Pathogenesis and Genetics Department, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, UK., Spiropoulos J; Neuropathology Section, Pathology Department, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, UK., Lockey R; Neuropathology Section, Pathology Department, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, UK., Tout AC; Molecular Pathogenesis and Genetics Department, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, UK., Jayasena D; Molecular Pathogenesis and Genetics Department, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, UK., Plater JM; Molecular Pathogenesis and Genetics Department, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, UK., Chave A; Molecular Pathogenesis and Genetics Department, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, UK., Green RB; Neuropathology Section, Pathology Department, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, UK., Simonini S; Neuropathology Section, Pathology Department, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, UK., Thorne L; Molecular Pathogenesis and Genetics Department, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, UK., Dexter I; Animal Services Unit, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, UK., Balkema-Buschmann A; Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 17493 Greifswald-Insel Riems, Germany., Groschup MH; Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 17493 Greifswald-Insel Riems, Germany., Béringue V; Virologie Immunologie Moléculaires, U892, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France., Le Dur A; Virologie Immunologie Moléculaires, U892, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France., Laude H; Virologie Immunologie Moléculaires, U892, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France., Hope J; Centre for Epidemiology and Risk Analysis, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, UK.
Jazyk:	angličtina
Zdroj:	The Journal of general virology [J Gen Virol] 2010 Aug; Vol. 91 (Pt 8), pp. 2132-2138. Date of Electronic Publication: 2010 Apr 14.
DOI:	10.1099/vir.0.018986-0
Abstrakt:	Twenty-four atypical scrapie cases from sheep with different prion protein genotypes from Great Britain were transmitted to transgenic tg338 and/or TgshpXI mice expressing sheep PrP alleles, but failed to transmit to wild-type mice. Mean incubation periods were 200-300 days in tg338 mice and 300-500 days in TgshpXI mice. Survival times in C57BL/6 and VM/Dk mice were >700 days. Western blot analysis of mouse brain samples revealed similar multi-band, protease-resistant prion protein (PrP(res)) profiles, including an unglycosylated band at approximately 8-11 kDa, which was shown by antibody mapping to correspond to the approximately 93-148 aa portion of the PrP molecule. In transgenic mice, the incubation periods, Western blot PrP(res) profiles, brain lesion profiles and abnormal PrP (PrP(Sc)) distribution patterns produced by the Great Britain atypical scrapie isolates were similar and compatible with the biological characteristics of other European atypical scrapie or Nor98 cases.
Databáze:	MEDLINE
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