Comparison of red blood cell membrane microstructure after different physicochemical influences: atomic force microscope research.

Autor: Moroz VV; V.A. Negovsky Research Institute of General Reanimatology, Russian Academy of Medical Sciences, Moscow, Russia., Chernysh AM, Kozlova EK, Borshegovskaya PY, Bliznjuk UA, Rysaeva RM, Gudkova OY
Jazyk: angličtina
Zdroj: Journal of critical care [J Crit Care] 2010 Sep; Vol. 25 (3), pp. 539.e1-12. Date of Electronic Publication: 2010 Apr 08.
DOI: 10.1016/j.jcrc.2010.02.007
Abstrakt: Purpose: After the influence of different actions on the blood, the erythrocytes may change their macrostructure. At the same time, the microstructure of cell membrane will be changed as well. This study provides the results of comparison of red blood cell membrane microstructure after they have been affected by different factors.
Materials and Methods: Images and spatial profiles of the cell surface were obtained by atomic force microscope. It was proposed to use spatial Fourier transform to decompose the initial complex profile into series of simple ones. This made it possible to compare surface parameters after exposure of red blood cells to different external actions.
Results: Quantitative differences between membrane profile harmonic composition parameters (amplitude and spatial period) after physical impact (impulse electrical field, osmotic swelling) and after chemical impact (the fixing fluid glutaraldehyde and the drug Esmeron) were experimentally confirmed.
Conclusions: Such experimental and theoretical approach may lay down the foundations of mechanisms of different factors' effect on red blood cells both in research and in clinics.
(Copyright © 2010 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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