| Autor: |
Phan PA; Translational Neuroscience Facility, Department of Physiology, School of Medical Sciences, University of New South Wales, Wallace Wurth Building, UNSW Kensington Campus, Sydney, NSW, 2052, Australia., Tadros SF, Kim Y, Birnbaumer L, Housley GD |
| Jazyk: |
angličtina |
| Zdroj: |
Histochemistry and cell biology [Histochem Cell Biol] 2010 Apr; Vol. 133 (4), pp. 437-48. Date of Electronic Publication: 2010 Mar 13. |
| DOI: |
10.1007/s00418-010-0686-x |
| Abstrakt: |
Canonical transient receptor potential type 3 (TRPC3) ion channels assemble from TRPC3 subunits and exhibit multiple activation mechanisms. TRPC3 has been proposed to contribute to Ca(2+) entry supporting Ca(2+) homeostasis in cochlear hair cells and to be activated by G protein-coupled receptor (GPCR) signaling in spiral ganglion neurons. The present study was designed to determine the spatiotemporal profile of TRPC3 expression during mouse cochlear ontogeny. TRPC3 immunofluorescence of cryosectioned cochleae was performed using E16-adult tissue. We found that prior to birth, TRPC3 expression was strongest in epithelial cells that form the cochlear partition. In the early postnatal period, to the onset of hearing (~P12), immunofluorescence was strongest in the hair cells, with increased expression in stria vascularis and Reissner's membrane. Afferent neurite labeling in inner spiral plexus and outer spiral bundles developed transiently in the perinatal period, corresponding to the critical period of synaptic consolidation, while signal in the spiral ganglion soma increased from the perinatal period through to adulthood. Compared with the late embryonic/early postnatal levels, hair cell expression was relatively weaker from the third postnatal week, whereas spiral ganglion soma labeling was stronger. In the adult, TRPC3 expression was primarily in the soma of spiral ganglion neurons, the hair cells, and the inner and outer sulcus regions. This spatiotemporal profile of TRPC3 expression was consistent with this ion channel contributing to development of sensory, neural and epithelial cochlear tissues, as well as hair cell Ca(2+) homeostasis and regulation of auditory neurotransmission via GPCR signaling. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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