| Autor: |
Fagundes A; Laboratório de Vigilância em Leishmanioses, Instituto de Pesquisa Clínica Evandro Chagas, Rio de Janeiro, RJ, Brasil. aline.fagundes@ipec.fiocruz.br, Schubach A, Paula CC, Bogio A, Antonio Lde F, Schiavoni PB, Monteiro Vde S, Madeira Mde F, Quintella LP, Valete-Rosalino CM, Vasconcellos Ede C, Azeredo-Coutinho RB, Pacheco RS, Marzochi MC, Marzochi KB |
| Jazyk: |
angličtina |
| Zdroj: |
Memorias do Instituto Oswaldo Cruz [Mem Inst Oswaldo Cruz] 2010 Feb; Vol. 105 (1), pp. 109-12. |
| DOI: |
10.1590/s0074-02762010000100018 |
| Abstrakt: |
The present study investigated the diagnostic value of polymerase chain reaction (PCR) performed in parallel to conventional methods at an American tegumentary leishmaniasis (ATL) referral centre for diagnosis. Accuracy parameters for PCR were calculated using 130 patients with confirmed ATL (ATL group), 15 patients established with other diseases and 23 patients with a lesion suggestive of ATL, but without parasitological confirmation (NDEF group). PCR showed 92.3% sensitivity, 93.3% specificity, a 99.2% positive predictive value and a 13.84 positive likelihood ratio. In the NDEF group, PCR confirmed ATL in 13 of the 23 patients, seven of whom responded to leishmaniasis treatment and six who presented spontaneous healing of the lesion. PCR should be included in the routine diagnostic procedures for ATL, especially for cases found to be negative by conventional methods. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
