| Autor: |
Huan TN; Department of Chemistry and Research Institute for Natural Sciences, Hanyang University, Seoul 133-791, Republic of Korea., Ha VT, Hung le Q, Yoon MY, Han SH, Chung H |
| Jazyk: |
angličtina |
| Zdroj: |
Biosensors & bioelectronics [Biosens Bioelectron] 2009 Oct 15; Vol. 25 (2), pp. 469-74. Date of Electronic Publication: 2009 Aug 07. |
| DOI: |
10.1016/j.bios.2009.08.002 |
| Abstrakt: |
A short chain peptide (16mer) has been successfully utilized for the selective electrochemical detection of the protein biomarker, protective antigen (PA), for the diagnosis of Anthrax. The major motivation of using a peptide instead of an antibody for the development of a biosensor is that there are advantages associated with the smaller size, better biological stability and easy synthesizability of a peptide. PA-selective peptide was synthesized and conjugated on a binding layer previously immobilized onto gold electrode. The same sensing scheme using a conventional antibody instead of a peptide was also tested for the purpose of comparison. Since the size of the peptide is approximately 1-2 orders of magnitude smaller than that of the antibody, a more populated immobilization of peptide on the sensing layer is possible and will eventually lead to improved sensitivity for PA detection. The selectivity of the peptide-based sensor was evaluated by observing the reduction peaks of samples containing PA with different concentrations of bovine serum albumin (BSA). The resulting responses were interference-free from BSA. The results of this study demonstrate the strong analytical potential of a peptide-based biosensor for diverse applications, especially in disease diagnosis where detection of a specific protein biomarker is particularly demanding. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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