Thermal stability of peroxidase from Chamaerops excelsa palm tree at pH 3.

kD, where k is a first-order kinetic constant that changes with temperature, as given by the Arrhenius equation; N is the native state, and D is the denatured state, and thermodynamic information was obtained by extrapolation of the kinetic transition parameters to an infinite heating rate. -->
Substance Nomenclature: EC 1.11.1.7 (Peroxidase)
Entry Date(s): Date Created: 20090512 Date Completed: 20090709 Latest Revision: 20090511
Update Code: 20231215
DOI: 10.1016/j.ijbiomac.2009.01.004
PMID: 19428462
Autor: Zamorano LS; Departamento de Química Física, Facultad de Ciencias Químicas, Universidad de Salamanca, 37008 Salamanca, Spain., Vilarmau SB, Arellano JB, Zhadan GG, Cuadrado NH, Bursakov SA, Roig MG, Shnyrov VL
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2009 May 01; Vol. 44 (4), pp. 326-32. Date of Electronic Publication: 2009 Jan 31.
DOI: 10.1016/j.ijbiomac.2009.01.004
Abstrakt: The structural stability of a peroxidase, a dimeric protein from palm tree Chamaerops excelsa leaves (CEP), has been characterized by high-sensitivity differential scanning calorimetry, circular dichroism and steady-state tryptophan fluorescence at pH 3. The thermally induced denaturation of CEP at this pH value is irreversible and strongly dependent upon the scan rate, suggesting that this process is under kinetic control. Moreover, thermally induced transitions at this pH value are dependent on the protein concentration, leading to the conclusion that in solution CEP behaves as dimer, which undergoes thermal denaturation coupled with dissociation. Analysis of the kinetic parameters of CEP denaturation at pH 3 was accomplished on the basis of the simple kinetic scheme N-->kD, where k is a first-order kinetic constant that changes with temperature, as given by the Arrhenius equation; N is the native state, and D is the denatured state, and thermodynamic information was obtained by extrapolation of the kinetic transition parameters to an infinite heating rate.
Databáze: MEDLINE
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