A rapid HPLC method used to establish pediatric reference intervals for vitamins A and E.

Autor: Johnson-Davis KL; Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, UT 84112, USA., Moore SJ, Owen WE, Cutler JM, Frank EL
Jazyk: angličtina
Zdroj: Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 2009 Jul; Vol. 405 (1-2), pp. 35-8. Date of Electronic Publication: 2009 Apr 08.
DOI: 10.1016/j.cca.2009.03.058
Abstrakt: Background: Serum concentrations of the fat-soluble vitamins A (retinol) and E (tocopherol) are measured to assess deficiency and, in the case of vitamin A, toxicity. We modified our existing HPLC method for analyzing vitamins A and E by using a high throughput analytical column and small diameter tubing to reduce analysis time. The modified HPLC method was used to establish pediatric reference intervals for these vitamins.
Methods: Serum or plasma proteins were precipitated with ethanol. A and E vitamins were extracted into hexane, evaporated under nitrogen, dissolved in absolute ethanol, and analyzed by HPLC with ultraviolet detection.
Results: The modified HPLC method correlated well with the existing method. Data analysis from the reference interval study resulted in age-dependent intervals for retinol and non-age-dependent intervals for retinyl palmitate, alpha-tocopherol, and gamma-tocopherol. Gender-based reference intervals were not necessary.
Conclusions: We validated a rapid HPLC method for analyzing vitamins A and E that decreased run-time by 60%, mobile phase consumption by 39%, and sample injection volume by 50%. The modified method was used to establish pediatric reference intervals for vitamins A and E in samples from 1136 healthy children aged 7 to 17 y.
Databáze: MEDLINE
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