| Autor: |
Enjalbert B; Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, UK., Moran GP, Vaughan C, Yeomans T, Maccallum DM, Quinn J, Coleman DC, Brown AJ, Sullivan DJ |
| Jazyk: |
angličtina |
| Zdroj: |
Molecular microbiology [Mol Microbiol] 2009 Apr; Vol. 72 (1), pp. 216-28. Date of Electronic Publication: 2009 Feb 23. |
| DOI: |
10.1111/j.1365-2958.2009.06640.x |
| Abstrakt: |
Candida albicans is more pathogenic than Candida dubliniensis. However, this disparity in virulence is surprising given the high level of sequence conservation and the wide range of phenotypic traits shared by these two species. Increased sensitivity to environmental stresses has been suggested to be a possible contributory factor to the lower virulence of C. dubliniensis. In this study, we investigated, in the first comparison of C. albicans and C. dubliniensis by transcriptional profiling, global gene expression in each species when grown under conditions in which the two species exhibit differential stress tolerance. The profiles revealed similar core responses to stresses in both species, but differences in the amplitude of the general transcriptional responses to thermal, salt and oxidative stress. Differences in the regulation of specific stress genes were observed between the two species. In particular, ENA21, encoding a sodium ion transporter, was strongly induced in C. albicans but not in C. dubliniensis. In addition, ENA21 was identified in a forward genetic screen for C. albicans genomic sequences that increase salt tolerance in C. dubliniensis. Introduction of a single copy of CaENA21 was subsequently shown to be sufficient to confer salt tolerance upon C. dubliniensis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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