| Autor: |
Jahnke K; Department of Neurology, Neuro-Oncology, and Blood-Brain Barrier Program, Oregon Health and Science University, Portland, OR, USA., Muldoon LL, Varallyay CG, Lewin SJ, Brown RD, Kraemer DF, Soussain C, Neuwelt EA |
| Jazyk: |
angličtina |
| Zdroj: |
Neuro-oncology [Neuro Oncol] 2009 Oct; Vol. 11 (5), pp. 503-13. Date of Electronic Publication: 2009 Jan 21. |
| DOI: |
10.1215/15228517-2008-119 |
| Abstrakt: |
To determine the efficacy of methotrexate and/or rituximab in a CNS lymphoma model and to evaluate MRI modalities for monitoring efficacy, we inoculated female athymic nude rats (rnu/rnu) intracerebrally with human MC116 B-lymphoma cells. Between days 16 and 26, rats were randomized to receive intravenous (IV) treatment with (1) saline (controls, n = 15), (2) methotrexate 1,000 mg/m(2) (n = 6), (3) rituximab 375 mg/m(2) (n = 6), or (4) rituximab plus methotrexate (n = 6). T2/fluid-attenuated inversion recovery (FLAIR) and gadolinium contrast-enhanced T1 MRI sequences were performed prior to and 1 week after treatment. IV rituximab gave an objective tumor response in four of six animals (>50% reduction in tumor volume comparing pre- and posttreatment T2/FLAIR MRI) and resulted in stable disease (50%-125% of baseline) in another animal. The percent change in tumor volume on T2/FLAIR images was significantly different in the control versus rituximab group (p = 0.0051). IV methotrexate slowed tumor growth, compared to controls, but only one of six animals had an objective response. In untreated controls, tumor histological volumes correlated well with T2/FLAIR or contrast-enhanced T1 images (r = 0.877). In the treatment groups, T2/FLAIR correlation was good, but the gadolinium-enhanced T1 MRI was not significantly correlated with histology (r = 0.19). The MC116 CNS lymphoma model seems valuable for preclinical testing of efficacy and toxicity of treatment regimens. IV rituximab was highly effective, but methotrexate was only minimally effective. T2/FLAIR was superior to contrast-enhanced T1 for monitoring efficacy. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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