| Autor: |
Braslavsky I, Amit R, Jaffar Ali BM, Gileadi O, Oppenheim A, Stavans J |
| Jazyk: |
angličtina |
| Zdroj: |
Applied optics [Appl Opt] 2001 Nov 01; Vol. 40 (31), pp. 5650-7. |
| DOI: |
10.1364/ao.40.005650 |
| Abstrakt: |
We introduce a method for detecting and tracking small particles in a solution near a surface. The method is based on blocking the backreflected illumination beam in an objective-type total internal reflection microscope, leaving unhindered the light scattered by the particles and resulting in dark-field illumination. Using this method, we tracked the motion of 60-nm polystyrene beads with a signal-to-noise ratio of 6 and detected 20-nm gold particles with a signal-to-noise ratio of 5. We illustrate the method's use by following the Brownian motion of small beads attached by short DNA tethers to a substrate. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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