Identification of a glucose permease from Mycobacterium smegmatis mc2 155.
| Autor: | Pimentel-Schmitt EF; Department of Microbiology, Friedrich Alexander University Erlangen-Nurnberg, Erlangen, Germany., Jahreis K, Eddy MP, Amon J, Burkovski A, Titgemeyer F |
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| Jazyk: | angličtina |
| Zdroj: | Journal of molecular microbiology and biotechnology [J Mol Microbiol Biotechnol] 2009; Vol. 16 (3-4), pp. 169-75. Date of Electronic Publication: 2008 Mar 03. |
| DOI: | 10.1159/000119546 |
| Abstrakt: | We report here the molecular identification of a glucose permease from Mycobacterium smegmatis,a model organism for our understanding of the life patterns of the major pathogens Mycobacterium tuberculosis and Mycobacterium leprae. A computer-based search of the available genome of M. smegmatis mc(2) 155 with the sequences of well-characterized glucose transporters revealed the gene msmeg4187 as a possible candidate. The deduced protein belongs to the major facilitator superfamily of proton symporters and facilitators and exhibits up to 53% of amino acid identity to other members of this family. Heterologous expression of msmeg4187 in an Escherichia coli glucose-negative mutant led to the restoration of growth on glucose. The determination of the biochemical features characterize MSMEG4187 (GlcP) as a high affinity (K(m) of 19 microM), glucose-specific permease. The results represent the first molecular characterization of a sugar permease in mycobacteria, and thus supply fundamental data for further in-depth analysis on the nutritional lifestyle of these bacteria. (Copyright 2008 S. Karger AG, Basel.) |
| Databáze: | MEDLINE |
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