| Autor: |
Winston SE; ADI Diagnostics, Rexdale, Ontario., Fuller SA, Evelegh MJ, Hurrell JG |
| Jazyk: |
angličtina |
| Zdroj: |
Current protocols in molecular biology [Curr Protoc Mol Biol] 2001 May; Vol. Chapter 11, pp. Unit11.1. |
| DOI: |
10.1002/0471142727.mb1101s50 |
| Abstrakt: |
Conjugation of enzymes to antibodies involves the formation of a stable, covalent linkage between an enzyme [e.g., horseradish peroxidase (HRPO), urease, or alkaline phosphatase] and an antigen-specific monoclonal or polyclonal antibody in which neither the antigen-combining site of the antibody nor the active site of the enzyme is functionally altered. This unit describes procedures for cross-linking HRPO, urease or alkaline phosphatase to immunoaffinity-purified monoclonal or polyclonal antibodies (IgG). |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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