| Autor: |
Pillai P; Department of Microbiology and Biotechnology Centre, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara 390002 Gujarat, India., Archana G |
| Jazyk: |
angličtina |
| Zdroj: |
Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2008 Mar; Vol. 78 (4), pp. 643-50. Date of Electronic Publication: 2008 Feb 09. |
| DOI: |
10.1007/s00253-008-1355-z |
| Abstrakt: |
Keratinases play an important role in biotechnological applications such as improvement of feather meal, enzymatic dehairing and production of amino acids or peptides from high molecular weight substrates. Bacillus subtilis P13, isolated from Vajreshwari hot spring (45-50 degrees C) near Mumbai, India, produces a neutral serine protease and has an optimum temperature of 65 degrees C. This enzyme preparation was keratinolytic in nature and could disintegrate whole chicken feathers, except for the remnants of shafts. The enzyme preparation also exhibited depilation of goat hides with the recovery of intact animal hair. The enzyme preparation could release peptides from ground feathers and bring about their weight reduction; however, similar action on hair was relatively weak. A single major PMSF-sensitive protease band could be detected upon zymogram analysis, indicating that a single enzyme may be responsible for feather degradation and hide depilation. The importance of these findings in the biotechnological application for feather and leather industries is discussed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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