| Autor: |
Bavis RW; Department of Biology, Bates College, Carnegie Science Hall, Lewiston, ME 04240, USA. rbavis@bates.edu, Wenninger JM, Miller BM, Dmitrieff EF, Olson EB Jr, Mitchell GS, Bisgard GE |
| Jazyk: |
angličtina |
| Zdroj: |
Respiratory physiology & neurobiology [Respir Physiol Neurobiol] 2008 Feb 29; Vol. 160 (3), pp. 301-12. Date of Electronic Publication: 2007 Oct 30. |
| DOI: |
10.1016/j.resp.2007.10.013 |
| Abstrakt: |
Perinatal hyperoxia attenuates the hypoxic ventilatory response in rats by altering development of the carotid body and its chemoafferent neurons. In this study, we tested the hypothesis that hyperoxia elicits this plasticity through the increased production of reactive oxygen species (ROS). Rats were born and raised in 60% O(2) for the first two postnatal weeks while treated with one of two antioxidants: vitamin E (via milk from mothers whose diet was enriched with 1000 IU vitamin E kg(-1)) or a superoxide dismutase mimetic, manganese(III) tetrakis (1-methyl-4-pyridyl) porphyrin pentachloride (MnTMPyP; via daily intraperitoneal injection of 5-10 mg kg(-1)); rats were subsequently raised in room air until studied as adults. Peripheral chemoreflexes, assessed by carotid sinus nerve responses to cyanide, asphyxia, anoxia and isocapnic hypoxia (vitamin E experiments) or by hypoxic ventilatory responses (MnTMPyP experiments), were reduced after perinatal hyperoxia compared to those of normoxia-reared controls (all P<0.01); antioxidant treatment had no effect on these responses. Similarly, the carotid bodies of hyperoxia-reared rats were only one-third the volume of carotid bodies from normoxia-reared controls (P <0.001), regardless of antioxidant treatment. Protein carbonyl concentrations in the blood plasma, measured as an indicator of oxidative stress, were not increased in neonatal rats (2 and 8 days of age) exposed to 60% O(2) from birth. Collectively, these data do not support the hypothesis that perinatal hyperoxia impairs peripheral chemoreceptor development through ROS-mediated oxygen toxicity. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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