[Validation of a simplified 13C-urea breath test method for the diagnosis of Helicobacter pylori infection].

Autor:	Ortiz-Olvera Nayeli NX; Universidad Autónoma del Estado de México, Mexico., Morán Villota S, Gallardo Wong I, Blancas Valencia JM, Cabrera Muñoz L
Jazyk:	Spanish; Castilian
Zdroj:	Revista espanola de enfermedades digestivas [Rev Esp Enferm Dig] 2007 Jul; Vol. 99 (7), pp. 392-7.
DOI:	10.4321/s1130-01082007000700005
Abstrakt:	Objective: To validate a simplified 13C-urea breath test (13C-UBT) method for the diagnosis of H. pylori infection. Material and Methods: Patients referred for gastrointestinal endoscopy and biopsy were included, and a 13C-UBT was performed after a 6-hour fast. Breath samples were collected in 10 ml glass tubes before and 30 min after the simultaneous administration of 50 mg of 13C-urea and 2 g of citric acid in 200 ml of water. All breath samples were analyzed using isotope ratio mass spectrometry. The diagnosis of H. pylori infection was established with a positive culture and/or positive histology and serology. Results: Eighty-eight patients were included, 49 female and 39 male with a mean age of 45 +/- 15 yrs. Fifty-one patients (57.95%) were positive and 30 (34.1%) negative for H. pylori. Seven cases (7.95%) were considered undetermined. The sensitivity, specificity, positive predictive value, and negative predictive value for 13C-UBT were 90.2, 93.3, 95.83, and 84.8%, respectively. Accuracy was 91.4%. Conclusions: The simultaneous administration of 50 mg of 13C-urea and 2 g of citric acid represents an alternative for the non-invasive diagnosis of H. pylori infection.
Databáze:	MEDLINE
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