| Autor: |
Polsky R; Biosensors & Nanomaterials, Sandia National Laboratories, P.O. Box 5800, MS-0892, Albuquerque, NM 87185, USA., Harper JC, Wheeler DR, Dirk SM, Arango DC, Brozik SM |
| Jazyk: |
angličtina |
| Zdroj: |
Biosensors & bioelectronics [Biosens Bioelectron] 2008 Jan 18; Vol. 23 (6), pp. 757-64. Date of Electronic Publication: 2007 Aug 23. |
| DOI: |
10.1016/j.bios.2007.08.013 |
| Abstrakt: |
The direct electrically addressable deposition of diazonium-modified antibodies is examined for electrochemical immunosensing applications. The immobilized antibodies can be detected by the use of electroactive enzyme tags and nanoparticle-gold labeling. Control over antibody functionalization density and minimal spontaneous grafting of diazonium-antibody adducts is shown. The utility of the technique for a sandwich immunoassay as well as the ability to individually and selectively address closely spaced microelectrodes for multi-target protein detection in an array format is demonstrated. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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