| Autor: |
Jahangir Tafrechi RS; Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands., van de Rijke FM, Allallou A, Larsson C, Sloos WC, van de Sande M, Wählby C, Janssen GM, Raap AK |
| Jazyk: |
angličtina |
| Zdroj: |
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society [J Histochem Cytochem] 2007 Nov; Vol. 55 (11), pp. 1159-66. Date of Electronic Publication: 2007 Aug 06. |
| DOI: |
10.1369/jhc.7A7282.2007 |
| Abstrakt: |
Segregation of mitochondrial DNA (mtDNA) is an important underlying pathogenic factor in mtDNA mutation accumulation in mitochondrial diseases and aging, but the molecular mechanisms of mtDNA segregation are elusive. Lack of high-throughput single-cell mutation load assays lies at the root of the paucity of studies in which, at the single-cell level, mitotic mtDNA segregation patterns have been analyzed. Here we describe development of a novel fluorescence-based, non-gel PCR restriction fragment length polymorphism method for single-cell A3243G mtDNA mutation load measurement. Results correlated very well with a quantitative in situ Padlock/rolling circle amplification-based genotyping method. In view of the throughput and accuracy of both methods for single-cell A3243G mtDNA mutation load determination, we conclude that they are well suited for segregation analysis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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