| Autor: |
Wei Q; Jinan University, School of Chemistry and Chemical Engineering, Jinan 250022, People's Republic of China (PRC)., Hongmin M, Duan C, Wang J, Liu S, Du B |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of AOAC International [J AOAC Int] 2007 Jan-Feb; Vol. 90 (1), pp. 238-43. |
| Abstrakt: |
The fluorescence intensity of the trihydroxyl-phenylfluorone-molybdenum(VI) [Mo(VI)] complex is quenched by protein. Based on this, a novel method for protein assay in aqueous solution was developed. With pH 3.75 acetic acid-sodium acetate buffer solution, in the presence of p-octyl polyethylene glycol phenyl ether microemulsion, the quenched fluorescence intensity is proportional to the concentration of bovine serum albumin (BSA) in the range of 0-7.00 microg/mL, and the detection limit of BSA is 5.65 ng/mL. There is no interference from amino acids and most metal ions. The method developed in this paper has been used for the successful determination of protein in human serum. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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