| Autor: |
Price JA 3rd; Department of Pathology, Oklahoma State University Center for Health Sciences, 1111 W. 17th. St. Tulsa, OK 74107-1898, USA. joseph.price@okstate.edu |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of biochemical and biophysical methods [J Biochem Biophys Methods] 2007 Apr 10; Vol. 70 (3), pp. 441-4. Date of Electronic Publication: 2006 Nov 10. |
| DOI: |
10.1016/j.jbbm.2006.10.008 |
| Abstrakt: |
Phospholipase A(2) is an important enzyme in various pathologies. Although fluorescent substrate assays for it have been recently developed, there is a need for an assay with inexpensive commercially available substrates, useful when samples interfered with fluorescent assays, that is nonisotopic, continuous, conducted at physiological pH, and in a 96 well format. A reaction using bromothymol blue was developed that meets all these requirements. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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