| Autor: |
Burnashev NA, Edwards FA, Verkhratskiĭ AN |
| Jazyk: |
ruština |
| Zdroj: |
Fiziologicheskii zhurnal [Fiziol Zh (1978)] 1991 Mar-Apr; Vol. 37 (2), pp. 119-22. |
| Abstrakt: |
Thin cardiac slices (100-200 microns) from newborn (1-14 days old) rat heart ventricles were used for patch clamp recordings. High resistance seals (10-50 GOhms) between patch-clamp pipettes and the membrane of cardiac cells as well as classical patch-clamp configurations can be achieved on this preparation without any enzymatic treatment of tissue. Resisting potential for cardiac cells measured in whole-cell configuration ranged between -30 and -65 mV. Averaged sodium currents and single inward rectifying potassium elementary currents recorded in cell-attached mode displayed basic features similar to those previously reported for isolated rat ventricular cells. Application of the method described here in cardiac electrophysiology will allow patch-clamp studies on heart cells without the complicated procedures of cell isolation. In addition, the uncertainty associated with enzyme treatment can be avoided. In future, this technique could be a new tool for studying electrophysiological properties of heart cells in situ. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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