Autor: |
Goryshin IIu, Tamm SE, Lantsov VA |
Jazyk: |
ruština |
Zdroj: |
Molekuliarnaia biologiia [Mol Biol (Mosk)] 1991 May-Jun; Vol. 25 (3), pp. 614-23. |
Abstrakt: |
The plasmid-transposon Tn9-322 was constructed by inverted transposition from the pBR322::Tn9 plasmid. The precise excision of the Tn9-322 transposon from the proB gene site can proceed by the Campbell's model. This fact was demonstrated by appearance of the plasmid-transposons after their precise excision. They contain two IS1 elements flanking a short direct repeat of the target DNA. The recombinational mechanism of precise excision of Tn9 type transposons seems not to be alternative but looks as an additional one to a well-known slippage mechanism proved for Tn5 and Tn10. |
Databáze: |
MEDLINE |
Externí odkaz: |
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