[Evidence for Campbell's mechanism for fine excision of Tn9 type transposons in Escherichia coli K12].

Autor: Goryshin IIu, Tamm SE, Lantsov VA
Jazyk: ruština
Zdroj: Molekuliarnaia biologiia [Mol Biol (Mosk)] 1991 May-Jun; Vol. 25 (3), pp. 614-23.
Abstrakt: The plasmid-transposon Tn9-322 was constructed by inverted transposition from the pBR322::Tn9 plasmid. The precise excision of the Tn9-322 transposon from the proB gene site can proceed by the Campbell's model. This fact was demonstrated by appearance of the plasmid-transposons after their precise excision. They contain two IS1 elements flanking a short direct repeat of the target DNA. The recombinational mechanism of precise excision of Tn9 type transposons seems not to be alternative but looks as an additional one to a well-known slippage mechanism proved for Tn5 and Tn10.
Databáze: MEDLINE