| Autor: |
Torres-Reverón A; Program in Neural and Behavioral Sciences, 450 Clarkson Ave, Box 29, Brooklyn, NY 11209, USA., Melnick SM, Stephenson SI, Dow-Edwards DL |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of neuroscience methods [J Neurosci Methods] 2006 Jan 30; Vol. 150 (2), pp. 143-9. Date of Electronic Publication: 2006 Jan 20. |
| DOI: |
10.1016/j.jneumeth.2005.05.018 |
| Abstrakt: |
In the traditional [14C] deoxyglucose (2DG) method for the measurement of local cerebral glucose utilization (LCGU), blood samples are collected from the femoral artery. However, the placement of a femoral catheter can affect locomotor activity of the animal. We wanted to develop a new technique for blood sampling that would not interfere with the ongoing behavior. Therefore, the present report establishes a method of collecting blood samples for the 2DG method through the jugular vein. To calibrate this method, catheters were inserted in both the femoral artery and jugular vein of adult male Sprague Dawley rats. The next day, rats were injected with 2DG (125 microCi/kg) through the jugular vein. To quantify 14C in plasma, the standard method of blood collection was used for the femoral artery while syringes were used to extract blood samples from the jugular vein. We calculated the integrated specific activity of the plasma and final tissue 2DG concentrations based on Sokoloff's original equation using blood samples derived from both vessels. LCGU determined in selected brain regions was equivalent using both sampling methods. In conclusion, sampling from the jugular vein is appropriate for the quantified 2DG method and does not disrupt locomotor activity of the rat. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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