| Autor: |
de Farias PM; Departamento de Biofísica e Radiobiologia - CCB, Federal University of Pernambuco, Cid. Universitária CEP 50670-901 Recife-PE, Brazil., Santos BS, de Menezes FD, Ferreira Rde C, Barjas-Castro Mde L, Castro V, Lima PR, Fontes A, Cesar CL |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of microscopy [J Microsc] 2005 Sep; Vol. 219 (Pt 3), pp. 103-8. |
| DOI: |
10.1111/j.1365-2818.2005.01501.x |
| Abstrakt: |
We report a new and efficient methodology of labelling red blood cells, in order to investigate the expression of anti-A antigen, employing luminescent semiconductor nanocrystals. Highly luminescent and stable core-shell cadmium sulphide/cadmium hydroxide [CdS/CdS(OH)2] colloidal particles were obtained in the nanometre size range. The surface of these particles was characterized by using a monoclonal anti-A antibody via a one-step glutaraldehyde cross-linking procedure, followed by conjugation of the particles to red cells of blood groups A+, and O+. Laser scanning confocal microscopy images indicated that after conjugation for 30 min, A+ and erythrocytes presented different patterns of dual bright emission whereas the O+ group cells showed no emission. We suggest that this labelling procedure may be applied as a quantitative tool to investigate the distribution and expression of alloantigen in red blood cells. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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