| Autor: |
Prasad PV; Department of Reproductive Biomedicine, National Institute of Health and Family Welfare, Munirka, New Delhi, India., Chaube SK, Shrivastav TG, Kumari GL, Duraiswami S, Muralidhar K |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of immunoassay & immunochemistry [J Immunoassay Immunochem] 2005; Vol. 26 (4), pp. 345-55. |
| DOI: |
10.1080/15321810500220985 |
| Abstrakt: |
Development of polyclonal antisera is still a choice in some hard-pressed budget laboratories. In the present study, an attempt was made to isolate alpha- and beta-subunits from peak-I hCG, generation of polyclonal antisera and their characterization. The anti-hCG-a antisera showed titres of 1: 8000 and anti-hCG-beta antisera 1: 16,000 at 50% binding to radiolabelled hCG in RIA. Studies on specificity using anti hCG-beta antiserum demonstrated no cross-reaction with several hormones tested in the present study, except for hCG-beta and hCG, thus eliciting a highly specific hCG-beta antiserum. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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