[ITS sequence identification of Radix Bupleuri].
| Autor: | Wu Y; School of Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beijing 100102, China. bwwy9851@163.com, Liu CS, Liu YF, Yan YN |
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| Jazyk: | čínština |
| Zdroj: | Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica [Zhongguo Zhong Yao Za Zhi] 2005 May; Vol. 30 (10), pp. 732-4. |
| Abstrakt: | Objective: To provide the basis of molecular authentication of Radix Bupleuri by the comparison of the internal transcribed spacer (ITS) sequences of five kinds of Radix Bupleuri in common use. Method: Firstly, total DNA of five kinds of Radix Bupleuri was extracted. Secondly, the ITS sequence was amplified by PCR with universal primer of ITS and PCR product was directly sequenced after purification. Result: The length of ITS1 and ITS2 sequence was 214-220 bp and 230-231 bp respectively. There were distinct variation sites between the ITS sequences of the five kinds of Radix Bupleuri. Conclusion: ITS sequence may be the evidence for the molecular authentication of Radix Bupleuri. |
| Databáze: | MEDLINE |
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