| Autor: |
Menozzi FD; Inserm, U629, Mécanismes Moléculaires de la Pathogénie Microbienne, Institut Pasteur de Lille, 1, rue du professeur Calmette, 59019 Lille cedex, France. franco.menozzi@ibl.fr, Reddy VM, Cayet D, Raze D, Debrie AS, Dehouck MP, Cecchelli R, Locht C |
| Jazyk: |
angličtina |
| Zdroj: |
Microbes and infection [Microbes Infect] 2006 Jan; Vol. 8 (1), pp. 1-9. Date of Electronic Publication: 2005 Apr 19. |
| DOI: |
10.1016/j.micinf.2005.03.023 |
| Abstrakt: |
Mycobacterium tuberculosis, the etiologic agent of tuberculosis, adheres to, invades and multiplies in both professional phagocytes and epithelial cells. Adherence to epithelial cells is predominantly mediated by the 28-kDa heparin-binding haemagglutinin adhesin (HBHA), which is also required for the extrapulmonary dissemination of the bacilli. To study the cellular mechanisms that might result in HBHA-mediated extrapulmonary dissemination, we used a transwell model of cellular barrier and fluorescence microscopy and found that HBHA induces a reorganization of the actin filament network in confluent endothelial cells, but does not affect the tight junctions that link them. When coupled to colloidal gold particles, HBHA-mediated a rapid attachment of the particles to the membrane of human laryngeal epithelial cells (non polarized HEp-2 cells) and human type II pneumocytes (polarized A-549 pneumocytes). After attachment, the particles were internalized in membrane-bound vacuoles that migrated across the polarized pneumocytes to reach the basal side. Attachment of the HBHA-coated particles was not observed when the epithelial cells were pretreated with heparinase III, a lyase that specifically cleaves the heparan sulfate chains borne by the proteoglycans. Furthermore, no binding was observed when the gold particles were coated with HBHA lacking its C-terminal heparin-binding domain. These observations indicate that HBHA induces receptor-mediated endocytosis through the recognition of heparan sulfate-containing proteoglycans by the heparin-binding domain of the adhesin. In addition, the transcellular migration of the endocytic vacuoles containing HBHA-coated particles suggests that HBHA induces epithelial transcytosis, which may represent a macrophage-independent extrapulmonary dissemination mechanism leading to systemic infection by M. tuberculosis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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