| Autor: |
Rocha HA; Laboratório de Glicobiologia, Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Rio Grande do Norte-UFRN, Natal-RN, Brazil. hugo-alexandre@uol.com.br, Bezerra LC, de Albuquerque IR, Costa LS, Guerra CM, de Abreu LD, Nader HB, Leite EL |
| Jazyk: |
angličtina |
| Zdroj: |
Planta medica [Planta Med] 2005 Apr; Vol. 71 (4), pp. 379-81. |
| DOI: |
10.1055/s-2005-864110 |
| Abstrakt: |
The brown seaweed Spatoglossum schröederi (Dictyotaceae) contains three main fucans (fucans A, B and C) with different mobility in electrophoresis. The fucan with highest mobility (fucan C) was precipitated with 2.0 volumes of acetone, purified using a combination of ion exchange chromatography and electrophoresis. It showed an MW of 24 kDa determined by HPLC and Sephadex G-75 chromatography and migrates as a single band in three distinct electrophoretic systems. This fucan contains fucose, xylose, galactose and sulfate in a molar ratio 1 : 0.6 : 2:2.3. The fucan has neither anticoagulant (from 10 to 100 microg) nor hemorrhagic activities (100 microg/mL). In addition, fucan C is neither cytotoxic nor cytostatic. However, fucan C (100 microg/mL) stimulated the synthesis of an antithrombotic heparan sulfate from endothelial cells of rabbit aorta. The results suggest that fucan C might be used as an antithrombotic therapeutic compound. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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