Comparison of COBAS AMPLICOR Neisseria gonorrhoeae PCR, including confirmation with N. gonorrhoeae-specific 16S rRNA PCR, with traditional culture.

Autor: Luijt DS; Regional Public Health Laboratory, van Ketwich Verschuurlaan 92, 9721 SW Groningen, The Netherlands. j.schirm@slgd.nl., Bos PA, van Zwet AA, van Voorst Vader PC, Schirm J
Jazyk: angličtina
Zdroj: Journal of clinical microbiology [J Clin Microbiol] 2005 Mar; Vol. 43 (3), pp. 1445-7.
DOI: 10.1128/JCM.43.3.1445-1447.2005
Abstrakt: A total of 3,023 clinical specimens were tested for Neisseria gonorrhoeae by using COBAS AMPLICOR (CA) PCR and confirmation of positives by N. gonorrhoeae-specific 16S rRNA PCR. The sensitivity of CA plus 16S rRNA PCR was 98.8%, compared to 68.2% for culture. Confirmation of CA positives increased the positive predictive value from 54.8 to 96.6%.
Databáze: MEDLINE