| Autor: |
Tong S; National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, MS-G17, Atlanta, GA 30333, USA. sot1@cdc.gov, Lingappa JR, Chen Q, Shu B, LaMonte AC, Cook BT, Birge C, Chern SW, Liu X, Galloway R, Mai le Q, Ng WF, Yang JY, Butany J, Comer JA, Monroe SS, Beard SR, Ksiazek TG, Erdman D, Rota PA, Pallansch MA, Anderson LJ |
| Jazyk: |
angličtina |
| Zdroj: |
The Journal of infectious diseases [J Infect Dis] 2004 Sep 15; Vol. 190 (6), pp. 1127-31. Date of Electronic Publication: 2004 Aug 17. |
| DOI: |
10.1086/422849 |
| Abstrakt: |
Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) emerged, in November 2002, as a novel agent causing severe respiratory illness. To study sequence variation in the SARS-CoV genome, we determined the nucleic acid sequence of the S and N genes directly from clinical specimens from 10 patients--1 specimen with no matched SARS-CoV isolate, from 2 patients; multiple specimens from 3 patients; and matched clinical-specimen/cell-culture-isolate pairs from 6 patients. We identified 3 nucleotide substitutions that were most likely due to natural variation and 2 substitutions that arose after cell-culture passage of the virus. These data demonstrate the overall stability of the S and N genes of SARS-CoV over 3 months during which a minimum of 4 generations for transmission events occurred. These findings are a part of the expanding investigation of the evolution of how this virus adapts to a new host. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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