[Cloning and expression of the gene for Escherichia coli thermostable enterotoxin].

Autor: Mikul'skis AV, Domoradskaia TI, Filippov SA, Dobrynin VN, Korobko VG
Jazyk: ruština
Zdroj: Bioorganicheskaia khimiia [Bioorg Khim] 1992 Jan; Vol. 18 (1), pp. 63-70.
Abstrakt: Gene estA coding for thermostable enterotoxin of Escherichia coli has been cloned. It is shown that in the E. coli strain SA162 this gene is located on the chromosome. Using polymerase chain reaction a site-directed mutagenesis of the cloned gene has been carried out, resulted in a recombinant strain--producer of the thermostable enterotoxin.
Databáze: MEDLINE