Standard operating procedure for immunuslotblot assay for analysis of DNA/sulfur mustard adducts in human blood and skin.

Autor: van der Schans GP; TNO Prins Maurits Laboratory, P.O. Box 45, 2280 AA Rijswijk, The Netherlands., Mars-Groenendijk R, de Jong LP, Benschop HP, Noort D
Jazyk: angličtina
Zdroj: Journal of analytical toxicology [J Anal Toxicol] 2004 Jul-Aug; Vol. 28 (5), pp. 316-9.
DOI: 10.1093/jat/28.5.316
Abstrakt: A standard operating procedure has been developed for an immunoslotblot assay of sulfur mustard adducts to DNA in human blood and skin for use in a field laboratory. A minimum detectable level of exposure of human blood in vitro (> or = 50 nM) sulfur mustard is feasible with the assay. In the case of human skin, a 1 s exposure to saturated sulfur mustard vapor (830 mg/m(-3)) could still be detected.
Databáze: MEDLINE