Molecular study on Y chromosome microdeletions in Egyptian males with idiopathic infertility.
| Autor: | El Awady MK; Department of Biomedical Technology, National Research Center, Dokki, Cairo 12622, Egypt. melawady@hotmail.com, El Shater SF, Ragaa E, Atef K, Shaheen IM, Megiud NA |
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| Jazyk: | angličtina |
| Zdroj: | Asian journal of andrology [Asian J Androl] 2004 Mar; Vol. 6 (1), pp. 53-7. |
| Abstrakt: | Aim: To determine the frequency of genetic deletions within the azoospermia factors in Egyptian infertile males. Methods: The Yq microdeletions in 33 infertile males with undetectable chromosomal anomalies were examined by mutiplex polymerase chain reaction (PCR). Deletions were confirmed using single PCR amplifications. Results: Four out of the total 33 (12 %) men had Yq(11) microdeletions, thus supporting the average reported figures in other populations. Three of those 4 cases had single short tandem sequence deletions with discrete histological findings of their testes. Single sY272 deletion within AZFc was associated with Sertoli cell only syndrome, whereas a patient with isolated sY84 deletion within AZFa had immature testicular structure. The remaining case had a large deletion in AZFa-c and short stature. Conclusion: The present study supports the hypothesis that the Yq(11) encompasses genetic determinants of stature besides genes controlling spermatogenesis. |
| Databáze: | MEDLINE |
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