| Autor: |
Li MQ; Department of Physics, University of California, Santa Barbara 93106., Hansma HG, Vesenka J, Kelderman G, Hansma PK |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of biomolecular structure & dynamics [J Biomol Struct Dyn] 1992 Dec; Vol. 10 (3), pp. 607-17. |
| DOI: |
10.1080/07391102.1992.10508671 |
| Abstrakt: |
Reproducible, high-contrast, nanometer-resolution AFM images of uncoated plasmid DNA can be obtained with nanogram quantities of DNA with the help of two advances in sample preparation: (1) Heating a DNA solution at 35 degrees C for 10 to 20 minutes before deposition on mica helps separate and spread the DNA, and (2) Using 5 microliter drops of the heated DNA solution in the concentration range of 2 to 10 nanogram/microliter in contact with a specially prepared mica surface for 5 to 10 minutes gives optimal coverage with only nanograms of DNA. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
