| Autor: |
Holloway PA; Department of Haematology, UMCU, Utrecht, The Netherlands., Kaldenhoven N, Kok-Schoemaker HM, Van Kessel B, Van Blokland WT, Bloem AC, Lokhorst HM |
| Jazyk: |
angličtina |
| Zdroj: |
Clinical and experimental immunology [Clin Exp Immunol] 2003 Feb; Vol. 131 (2), pp. 340-6. |
| DOI: |
10.1046/j.1365-2249.2003.02067.x |
| Abstrakt: |
Cytotoxic T cells play an important role in graft-versus-host-disease (GvHD) and graft-versus-leukaemia/myeloma, which may occur in patients treated with an allogeneic stem cell transplantation (ASCT). Here, we describe the selection of a myeloma reactive CD4+ cytotoxic T cell-line (CTL) and two CD4+ clones from this CTL. The CTL was generated from the blood from a patient with multiple myeloma (MM) with graft versus myeloma/GvHD, following an ASCT. The CTL was stimulated using irradiated peripheral blood mononuclear cells and EBV transformed B cells from the myeloma patient (EBVp), both of which were obtained prior to ASCT. Both the CTL and the two T cell clones specifically lysed EBVp and secreted IFN-gamma after coculture with EBVp and autologous myeloma tumour cells in a class II restricted fashion. These results show that myeloma tumour cells and autologous B cells present a common polymorphic peptide that functions as a target for graft derived cytotoxic T cells. Identification of these proteins will give insight into the relationship between graft versus myeloma (GvM) and GvHD and may provide immunotherapeutical targets in the treatment of MM. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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