| Autor: |
Pendergrass JA Jr; Armed Forces Radiobiology Research Institute, Radiation Medicine Department, Bethesda, MD 20889-5603, USA. pendergrass@afrri.usuhs.mil, Srinivasan V, Kumar KS, Jackson WE 3rd, Seed TM |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of AOAC International [J AOAC Int] 2002 May-Jun; Vol. 85 (3), pp. 551-4. |
| Abstrakt: |
A liquid chromatographic method using electrochemical detection is presented for measuring the thiol WR-1065 12-(3-aminopropylamino) ethanethiol] and its symmetrical disulfide WR-33278 [NH2(CH2)3NHCH2CH2S]2. WR- 1065 is the active, radioprotective drug derived from the phosphorothioate pro-drug WR-2721 (amifostine). External standard curves for both compounds were linear over the range of 40-200 pmol injected (r2 = 0.999 and 0.996 for the thiol and disulfide, respectively). The detection and quantitation limits for WR-1065 were 9 and 18 pmol, respectively, whereas the corresponding values for WR-33278 were 30 and 59 pmol, respectively. Within- and between-day determinations of measurement Vision and accuracy for both compounds validated the suitability of this assay method. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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