| Autor: |
Mutoloki S; National Institute of Animal Health, Kamikita, Aomori, Japan., Ishii M, Narita M |
| Jazyk: |
angličtina |
| Zdroj: |
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire [Can J Vet Res] 2002 Jan; Vol. 66 (1), pp. 60-3. |
| Abstrakt: |
Bovine herpesvirus type 1 (BHV-1) and Mannheimia haemolytica antigens were demonstrated in lung tissues that were stored in 10% neutral phosphate buffered formalin for 1 to 22 months using the immunoperoxidase method. There were no differences observed in terms of labelling intensity and distribution of M. haemolytica antigens between specimens stored for 1 and 22 months. The labeling intensity in sections from 2-cm thick specimens was comparable to those from 0.2-cm thick specimens. There was no difference observed between pronase-treated and -untreated sections. However, for BHV-1, the labeling intensity in untreated sections was reduced in tissues that had been stored from 12 to 22 months. Sections from thin specimens stored in neutral buffered formalin for 22 months exhibited a stronger staining intensity than those from thick specimens. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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