| Autor: |
Bourreau E; Immunologie des Leishmanioses, Institut Pasteur de la Guyane Française, BP 6010, 97304 Cayenne, French Guiana., Collet M, Prévot G, Milon G, Ashimoff D, Hasagewa H, Parra-Lopez C, Launois P |
| Jazyk: |
angličtina |
| Zdroj: |
European journal of immunology [Eur J Immunol] 2002 Feb; Vol. 32 (2), pp. 510-20. |
| DOI: |
10.1002/1521-4141(200202)32:2<510::AID-IMMU510>3.0.CO;2-4 |
| Abstrakt: |
Leishmania guyanensis (L.g.)-specific CD8+ T cells can be isolated from PBMC of subjects who have never been previously exposed to Leishmania. Cells that produce IFN-gamma in response to live L.g. are generated from naive CD45RA+CD8+ T cells. The generation of L.g.-specific CD8+ T cells requires the presence of whole L.g. or UV-irradiated parasite but not the soluble antigens from L.g. promastigotes. The IFN-gamma-producing T cells recognize a specific antigen, the Leishmania homologue of receptors of activated C kinases (LACK) and this antigen but not live L.g. can produce a strong IL-10 response in CD45RA-CD4+ memory T cells from naive subjects. A single epitope (amino acid 156-173) is found to induce the IL-10 synthesis. While the IFN-gamma-producing cells are present among CD45RA+CD8+ T cells that are CD62L-CDR7- and CLA-, the LACK-reactive IL-10-producing cells are CD4+ T cells that are CD62L+CCR7- and CLA-. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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