| Autor: |
Susloparov IM, Pliasunov IV, Safronov PF, Bakhtina MM, Lokteva LA, Grishaev MP, Susloparov MA |
| Jazyk: |
ruština |
| Zdroj: |
Molekuliarnaia genetika, mikrobiologiia i virusologiia [Mol Gen Mikrobiol Virusol] 2001 (2), pp. 34-7. |
| Abstrakt: |
The most potent antigen among HSV-1 proteins are glycoproteins gB(UL27) and gD(US6). Multiple amino acid sequence alignment of these proteins shows that gD protein is the most specific for HSV-1. Analysis of gD protein epitopes detected the main antigenic determinants not cross-reactive with antigens of other viruses. Virus was isolated and genome DNA was prepared from morphological elements of a patient with herpes simplex infection. US6 gene fragment was cloned in pUC19 vector. Cloning in bacterial expression vectors helped obtain beta-galactosidase-fused recombinant HSV-1 gD protein with 6-histidines affine target for high-performance chromatography purification. ELISA with a set of HSV-1-positive and negative donor sera and a commercial panel of HSV-1 sera (Vektor-Best) showed that recombinant gD can be used as an antigen to HSV-1-specific IgG. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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