| Autor: |
Rodrigues JL; NSF Center for Microbial Ecology, Department of Crop and Soil Sciences, and Institute for Environmental Toxicology, Michigan State University, East Lansing, Michigan 48824, USA., Maltseva OV, Tsoi TV, Helton RR, Quensen JF 3rd, Fukuda M, Tiedje JM |
| Jazyk: |
angličtina |
| Zdroj: |
Environmental science & technology [Environ Sci Technol] 2001 Feb 15; Vol. 35 (4), pp. 663-8. |
| DOI: |
10.1021/es001308t |
| Abstrakt: |
The Gram-positive bacterium Rhodococcus sp. strain RHA1, naturally containing the biphenyl pathway, was electroporated with a broad host range plasmid containing the 4-chlorobenzoate (4-CBA) degradation operon (fcb) isolated from Arthrobacter globiformis strain KZT1. The recombinant strain grew in medium containing 4-CBA and 4-chlorobiphenyl (4-CB) as the only source of carbon, with stoichiometric release of chloride and a molar growth yield on 4-CB that suggested utilization of both biphenyl rings. In resting cell assays, similar rates of degradation were observed for wild-type and recombinant strains for the most common eight congeners from the anaerobic dechlorination of Aroclor 1242, but the recombinant strain accumulated lower amounts of chlorinated meta-cleavage products and no 4-CBA. Recombinant cells inoculated at 10(4) cells/g into nonsterile soil amended with 4-CB grew to 6-10(5) cells/g, a density consistent with the 4-CB consumed. 4-CB was removed only in the inoculated soil, and the recombinant strain did not grow in the same soil when it was not amended with 4-CB. The fcb operon remained stable in the recombinant strain reisolated from soil after 60 days. This work provides proof of concept that a Rhodococcus strain constructed to grow on a PCB would grow in nonsterile soil if the appropriate chlorobiphenyl is available. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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